Today performed Plasmid isolation.
Having performed this practical quite a number of times at college, felt familiar with the procedure and handling of the work. Ah! it feels good.
While performing the practical, had a great conversation with Vinita Sharma Di.(JRF).
We usually quantify the amount of DNA by measuring it's UV absorption. But why don't we follow the same for protein estimation? Ever wondered? That's because only 3 amino acids are known to absorb UV light and they are, Tyrosine, Tryptophan and Phenylalanine. Since, all the amino acids comprising the protein do not absorb UV, it doesn't give proper estimation. Therefore, other methods are usually used.
The DNA absorbs UV at 260-280 nm. Maximum at 280nm.
The purity of DNA is determined by the A260/A280 ratio of 1.7-2.0
But the main question comes, why does any bio molecule, let's say, DNA, absorb UV?
DNA is a very complex molecule electronically, the part of the molecule that is thought to absorb most of the UV radiation is the aromatic ring of Purine and pyrimidine bases. As we know, there are different energy states in an atom and when an electron jumps from one energy level to another, there is either release or absorption of energy. So, in the DNA molecule, the difference between two energy states of DNA structure matches with the energy of a UV photon and hence the UV radiation is absorbed.
Find out more: Link_1 Link_2
Another Day wraps up. Day 12 Good bye.
Having performed this practical quite a number of times at college, felt familiar with the procedure and handling of the work. Ah! it feels good.
While performing the practical, had a great conversation with Vinita Sharma Di.(JRF).
We usually quantify the amount of DNA by measuring it's UV absorption. But why don't we follow the same for protein estimation? Ever wondered? That's because only 3 amino acids are known to absorb UV light and they are, Tyrosine, Tryptophan and Phenylalanine. Since, all the amino acids comprising the protein do not absorb UV, it doesn't give proper estimation. Therefore, other methods are usually used.
The DNA absorbs UV at 260-280 nm. Maximum at 280nm.
The purity of DNA is determined by the A260/A280 ratio of 1.7-2.0
But the main question comes, why does any bio molecule, let's say, DNA, absorb UV?
DNA is a very complex molecule electronically, the part of the molecule that is thought to absorb most of the UV radiation is the aromatic ring of Purine and pyrimidine bases. As we know, there are different energy states in an atom and when an electron jumps from one energy level to another, there is either release or absorption of energy. So, in the DNA molecule, the difference between two energy states of DNA structure matches with the energy of a UV photon and hence the UV radiation is absorbed.
Find out more: Link_1 Link_2
Another Day wraps up. Day 12 Good bye.
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