Day 52:

Today,

From the results of polymorphic survey of markers and genotyping of mapping population, we did scoring of the gel images. 

Scoring is comparing each individual of the mapping population with it's two parents. The progeny resembling parent 1 will be named A and that resembling parent 2 will be named B, the heterozygotes that have inherited from both the parents are scored as H. 

This scoring is done for each individual marker that was identified to be polymorphic in the parents.

Later the data obtained, will be used for construction of linkage map.

fig. gel image of genotyping of mapping population (94 individuals) with a specific marker

In the gel image above, observe the bands of P1 and P2 in row 1 and row 3. The progeny resembling the parents or being a hybrid of both parents can be identified easily. Example, progeny 11, 13, 14, 15, 18 and 22,etc resemble parent 1, while progeny 1, 2, 3, 5, 6,etc resemble parent 2.