Today's task was about estimating amylose from approximately 50 starch samples of wheat.
1. Plotting standard curve
2. Estimating amylose content from starch samples.
- starch sample does not dissolve in water. So, DMSO (Dimethyl Sulphonate) is used as solvent
- Then the samples are allowed to rest for 10 minutes in boiling water bath at 85C
- Amylose content is estimated by using Spectrophotometry
- Iodine is used in the process for estimation because Starch- Iodine complex gives a blue-violet coloration which can be detected using spectrophotometer at 620 nanometer.
A very long and tiring day from 9:00 am to 10:30 pm. But at the end, the results weren't satisfactory. Spectrophotometer probably got too tired and collapsed because it was continually under use for past two days. Hoping for good start tomorrow.
Good night.
1. Plotting standard curve
2. Estimating amylose content from starch samples.
- starch sample does not dissolve in water. So, DMSO (Dimethyl Sulphonate) is used as solvent
- Then the samples are allowed to rest for 10 minutes in boiling water bath at 85C
- Amylose content is estimated by using Spectrophotometry
- Iodine is used in the process for estimation because Starch- Iodine complex gives a blue-violet coloration which can be detected using spectrophotometer at 620 nanometer.
A very long and tiring day from 9:00 am to 10:30 pm. But at the end, the results weren't satisfactory. Spectrophotometer probably got too tired and collapsed because it was continually under use for past two days. Hoping for good start tomorrow.
Good night.
No comments:
Post a Comment