After performing DNA iolation, PCR, restriction digestion or ligation or any other experiment involving DNA and RNA, analyzing the gel becomes an important feature.
In the following gels, one can detect polymorphic or monomorphic nature of the primer being used.
In this gel, Primers are used to check for polymorphism in 2 parents P1 and P2. If a primer generates bands of different sizes in Parent1 and parent 2, it is known to be polymorphic.
It can be observed that Primer1, 2, 3,5,6 show polymorphism and can be used as markers.
The 9th well represents the DNA Ladder.
Polymorphism refers to a condition where there is variation(difference) in the particular sequence amplified by the primer.
In this gel, the primer used for 30 DNA samples is monomorphic as the bands all lie in the same size portion. It means the primer amplified sequences similar in all the samples.
In the following gels, one can detect polymorphic or monomorphic nature of the primer being used.
It can be observed that Primer1, 2, 3,5,6 show polymorphism and can be used as markers.
The 9th well represents the DNA Ladder.
Polymorphism refers to a condition where there is variation(difference) in the particular sequence amplified by the primer.
In the above gel a single primer was used in 34 samples. It shows polymorphism in DNA of each sample amplified by the Primer.
In this gel, the primer used for 30 DNA samples is monomorphic as the bands all lie in the same size portion. It means the primer amplified sequences similar in all the samples.
No comments:
Post a Comment