The day started with exchanging pleasant Good mornings at the lab.
Here are a few standard operating procedures we follow at the Lab:
1. Before using any equipment at the lab, the researcher has to pre-book it by making an entry in the logbook provided for each instrument. After using the instrument, the log-out time has to be entered.
2. Before starting an experiment, the details of it (Ex- procedure, chemicals being used, the serial numbers of the samples, etc) should be noted down in the Notebook provided for each researcher.
A daily report is expected in the notebook with images of results if any.
3. A proper prior planning of the experiment being carried out is essential. The errors that might arise during the experiment should be pre-known.
Example: If the parents are being screened for polymorphic primers, all the primer names are noted down in order. Then in the same order, they are provided in the 96-well plate for performing PCR. This makes it easier to analyze the results later.
4. All the calculations regarding the experiment should be carried out in the researchers notebook.
5. Gloves and Lab-coat are essential while handling chemicals and solutions.
6. The working area should be properly cleaned with 70% ethanol before and after use.
7. There are separate working areas for writing work, preparing solutions or reaction mixtures, preparing gels which involves use of ethidium bromide, etc.
8. Disposal of wastes are categorized into plastics/ papers, SDS- PAGE/ Agarose gels, liquid wastes, hazardous wastes and miscellaneous wastes.
9. Discard mugs are handy and should be used while pipetting as it requires frequent disposal of micro tips.
10. The solutions should be stored as necessary, either at RT, -20C, -4C or 4C.
11. The glassware and used tubes should be placed at washing area after use.
12. Area around the instrument should be cleaned properly after use and care should be taken in handling the instruments.
13. Standardize the instrument before use. If using weighing balance, pipettes, etc, make sure they are properly calibrated.
14. Standard glassware or measuring cylinders should be used while preparing solutions.
15. Ensure keeping all the equipment or glassware in their respective places after use.
16. Photo reactive solutions/ chemicals should be stored in amber colored bottles.
17. SOP of each Instrument should be properly studied before using it.
18. A properly planned experiment always saves time, energy and efforts.
19. Proper labelling of chemicals, reactions, PCR plates, samples,etc are a must
20. Greet everyone with a smile. It makes the work environment more live and pleasant.
Here are a few standard operating procedures we follow at the Lab:
1. Before using any equipment at the lab, the researcher has to pre-book it by making an entry in the logbook provided for each instrument. After using the instrument, the log-out time has to be entered.
2. Before starting an experiment, the details of it (Ex- procedure, chemicals being used, the serial numbers of the samples, etc) should be noted down in the Notebook provided for each researcher.
A daily report is expected in the notebook with images of results if any.
3. A proper prior planning of the experiment being carried out is essential. The errors that might arise during the experiment should be pre-known.
Example: If the parents are being screened for polymorphic primers, all the primer names are noted down in order. Then in the same order, they are provided in the 96-well plate for performing PCR. This makes it easier to analyze the results later.
4. All the calculations regarding the experiment should be carried out in the researchers notebook.
5. Gloves and Lab-coat are essential while handling chemicals and solutions.
6. The working area should be properly cleaned with 70% ethanol before and after use.
7. There are separate working areas for writing work, preparing solutions or reaction mixtures, preparing gels which involves use of ethidium bromide, etc.
8. Disposal of wastes are categorized into plastics/ papers, SDS- PAGE/ Agarose gels, liquid wastes, hazardous wastes and miscellaneous wastes.
9. Discard mugs are handy and should be used while pipetting as it requires frequent disposal of micro tips.
10. The solutions should be stored as necessary, either at RT, -20C, -4C or 4C.
11. The glassware and used tubes should be placed at washing area after use.
12. Area around the instrument should be cleaned properly after use and care should be taken in handling the instruments.
13. Standardize the instrument before use. If using weighing balance, pipettes, etc, make sure they are properly calibrated.
14. Standard glassware or measuring cylinders should be used while preparing solutions.
15. Ensure keeping all the equipment or glassware in their respective places after use.
16. Photo reactive solutions/ chemicals should be stored in amber colored bottles.
17. SOP of each Instrument should be properly studied before using it.
18. A properly planned experiment always saves time, energy and efforts.
19. Proper labelling of chemicals, reactions, PCR plates, samples,etc are a must
20. Greet everyone with a smile. It makes the work environment more live and pleasant.
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